HPLC-MS/MS Determination of 13 N-Nitrosamines in Elastomeric Material of Toys

HPLC-MS/MS was applied to the determination of 13 N-nitrosamines in elastomers of toys. The sample was extracted with simulated saliva. Agilent Eclipse XDB-C18 chromatographic column was used as stationary phase,and mixtures of 0.1% (φ) formic acid aqueous solution and 0.1% (φ) formic acid methanol solution mixed in various ratio were used as mobile phase in gradient elution. ESI+ and selected reaction monitoring mode were adopted in MS/MS. Linear relationships between values of peak area and mass fraction of 13 N-nitrosamines were kept in the same range of 1-50 μg·kg-1,with detection limits (3s/k) in the range of 0.012-0.138 μg·kg-1. Tests for recovery were made by standard addition method at the concentration levels of 1,5,50 μg·kg-1,giving values of recovery and RSD′s (n=6) in the ranges of 77.4%-113% and 4.0%-12%,respectively.